Results showed that the VEGFA mRNA and protein expression in heart, liver and kidney of TP was higher than that in YH and YL. Quantitative real-time PCR and western blots were used to measure expression levels and PCR products were sequenced. We used PCR and Sanger sequencing to screen for single nucleotide polymorphisms (SNPs) in 5สน-flanking DNA and exons of the VEGFA gene. In this study, we examined VEGFA gene expression in the heart, liver, and kidney of Tibetan pigs (TP), Yorkshire pigs that migrated to high altitudes (YH), and Yorkshire pigs that lived at low altitudes (YL). These processes play key roles in physiological blood vessel formation and pathological angiogenesis. Vascular endothelial growth factor A ( VEGFA) can induce endothelial cell proliferation, promote cell migration, and inhibit apoptosis.
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